CBSE Questions for Class 12 Medical Biology Biotechnology: Principles And Processes Quiz 7 - MCQExams.com

Modified tumour free $$T_1$$ plasmid carrying a desired gene is called
  • Cosmid
  • Phagemid
  • T-DNA
  • BAC
The plasmid of which organism was used successfully for the first time as vector by Cohen and 
  • Boyer
  • Salmonella typhimurium
  • Streptococcus pneumoniae
  • Streptococcus aureus
  • Rhizobium leguminosarum
In plasmid pBR322, BR stands for
  • Baculovirus and Retrovirus
  • Boyer and Reed
  • Bollivar and Rodrigues
  • Bacillus and Rhizobium
Agrobacterium tumefaciens is most widely used for gene transfer because of/ as 
  • It causes crown gall tumours
  • Its ability to insert Ti plasmid into nuclear genome
  • It can grow anywhere
  • It has the ability to kill pathogenic bacteria
Restriction endonucleases are obtained from 
  • Bacteria
  • All prokaryotic cells
  • Bacteriophages
  • Plasmids
Which of the following is not component of downstream processing ____________.
  • Expression
  • Separation
  • Purification
  • Preservation
Which one of the following statements is not correct about a plasmid?
  • It is a circular DNA.
  • It has the ability of autonomous replication.
  • Its DNA is as long as chromosonal DNA.
  • It has antibiotic resistant gene.
Which of the following does not produce sticky ends?
  • Sma I
  • EcoRI
  • SalI
  • BamHI
Taq polymerase used in polymerase chain reaction is active at temperature of
  • 25$$^o$$ - 30$$^o$$ C
  • 35$$^o$$ - 50$$^o$$ C
  • 50$$^o$$ - 70$$^o$$ C
  • 72$$^o$$ - 90$$^o$$C
Most suitable method of introducing alien DNA into a plant cell is
  • Biolistics
  • Micro-injection
  • Lipofection
  • Heat shock method
Match the List-I with List-II and select the correct answer.
List-IList-II
a. Recombinant DNA$$1$$. Marker gene-reporter gene
b. Agro bacterium$$2$$. DNA lipase
c. Sticky ends$$3$$. T-DNA
d. Plasmids$$4$$. Restriction enzymes
  • A-$$3$$, B-$$4$$, C-$$1$$, D-$$2$$
  • A-$$3$$, B-$$4$$, C-$$2$$, D-$$1$$
  • A-$$4$$, B-$$3$$, C-$$1$$, D-$$2$$
  • A-$$4$$, B-$$3$$, C-$$2$$, D-$$1$$
Ti plasmid used in genetic engineering is obtained from _________________.
  • Bacillus thuringiensis
  • Agrobacterium rhizogenes
  • Agrobacterium tumefaciens
  • Pseudomonas syringae
Which one of the following is NOT a PCR-based molecular marker?
  • RAPD
  • AP-PCR
  • DAF
  • RELP
Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?
  • $$1, 3, 2, 4, 5$$
  • $$3, 2, 1, 5, 4$$
  • $$1, 3, 5, 4, 2$$
  • $$5, 1, 3, 4, 2$$
Find the correct statement with respect to function or properties of RNA polymerase.
  • Bacteria have $$3$$ different types of RNA polymerase
  • RNA polymerase binds to promoter and initiate transcription
  • It uses nucleoside monophosphate as substrate
  • RNA polymerase polymerizes in a template independent manner
Thermal cycler is used in one of these reactions.
  • Radioactivity
  • Enzyme catalysed reaction
  • Chemical reaction
  • Polymerase chain reaction
Restriction enzymes cut DNA at specific sites known as ______________.
  • Telomeric sequences
  • Palindromic sequences
  • Terminator sequences
  • Attenuator sequences
The gene promoter sequences, commonly used in gene cloning experiments are derived from __________________.
  • TMV(Tobacco Mosaic Virus)
  • TSWV(Tomato Spotted Wilt Virus)
  • CMV(Cucumber Mosaic Virus)
  • CaMV(Cauliflower Mosaic Virus)
The most commonly used method of choice for gene transfer using immature embryos as explants is ______________.
  • Microprojectile
  • Electroporation
  • Liposome medfeted
  • Chemically stimulated
Which of the following characteristics make the end nuclease a restriction enzyme?
a. Restriction infection of bacteria by certain viruses(bacteriophages) by degrading the viral DNA.
b. Ability to cut only the double-stranded DNA
c. Ability to cut DNA into specific fragments containing identifiable genes, at any position.
Select the correct answer using the codes given.
  • $$1$$ and $$2$$
  • $$1$$ and $$3$$
  • $$2$$ and $$3$$
  • $$1, 2$$ and $$3$$
Changes in transcription after linking the cloned DNA of the putative promoter/enhancer region to cloned DNA of the coding region of an unrelated gene, can be measured rapidly with a high degree of sensitivity using.
  • Transgenic technology
  • Reporter gene technology
  • cDNA technology
  • Gene transfer technology
Which one of the following viruses has single stranded DNA and is used extensively as cloning vector in recombinant DNA technology?
  • TMV
  • Lambda phage
  • Adenovirus
  • $$M_{13}$$
A shuttle vector is defined as _______________.
  • A plasmid capable of replicating in one orgasm
  • A plasmid capable of accepting only large DNA inserts
  • A plasmid incapable of replicating in cells
  • A plasmid capable of replicating in two different organisms
DNA is amplified by __________________.
  • PCR technique
  • Southern Blotting technique
  • Northern Blotting technique
  • Nucleic acid hybridization technique
Who of the following is associated with the discovery of 'Restriction endonuclease'.
  • Hamilton Smith
  • H. G. Khorana
  • Marshall Nirenberg
  • S. B. Weiss
With reference to the designing of vectors based on Ti and Ri plasmids, consider the following statements.
a. They are disarmed by removing the genes causing the disease
b. They carry sites for insertion of gene of interest.
c. They carry selectable markers.
Which of the statements given are correct?
  • $$1$$ and $$2$$ only
  • $$2$$ and $$3$$ only
  • $$1$$ and $$3$$ only
  • $$1, 2$$ and $$3$$
Which one of the following is the method of gene transfer based on the use of short electrical impulses of high field strength?
  • Ultrasoniation
  • Electroporation
  • Microprojectile
  • Macro injection
The plasmid derived from E. Coli plasmid Col EI is?
  • YIP
  • pYAC
  • pBR-$$322$$
  • Ti-plasmid
Which one of the following enzymes is used in PCR reaction?
  • Taq polymerase
  • RNA polymerase
  • Rib nuclease
  • End nuclease
How many cycles of PCR are required if desired number of DNA molecules is 64 and starting material is single stranded DNA instead of ds DNA ?
  • 5
  • 64
  • 7
  • 30
When E coli with DNA $$^{15}N ^{15}N $$ is allowed to grow in a medium containing $$^{14}N$$ the number of DNA strands with $$^{15}N$$ after five generations is: 
  • 1
  • 2
  • 8
  • 16
  • 30
A temperate bacteriophage.
  • Causes complete bursting of the infected bacterial cell after the latent period
  • Does not cause bursting of the infected bacterial cell after the latent period
  • Does not cause the death of the infected bacterial cell but continues to exit there and this cell divides almost like a normal cell
  • Does not cause the death of the infected bacterial cell but the infected cell ceases to divide
Polymerase chain reaction technique is used for.
  • DNA amplification
  • Screening gene libraries
  • Synthesis of polymerase
  • DNA sequencing
When a sample containing antibody $$1\ gM$$ is run on SDS - PAGE, what molecular weight bands are expected to be seen?
  • $$70, 55. 15\ Kbp$$
  • $$50, 15\ Kbp$$
  • $$50, 55\ Kbp$$
  • $$50, 25\ Kbp$$
Bacterial colonies that have a foreign DNA fragment inserted in to the plasmid will appear white because:
  • X-gal can be cleaved by $$\beta$$- galactoxidase
  • Gal gene shows insertional inactivation due to insertion of foreign DNA
  • Gal gene is active
  • X-gal can be cleaved by permease
Which of the following techniques can be used to detect genetic disorders in humans?
  • PCR
  • Gel electrophoresis
  • Chromatography
  • Spectroscopy
Which one of the following is not essential for polymerase chain reaction (PCR)?
  • Restriction enzyme
  • Denaturation of DNA
  • Primers
  • DNA polymerase
Insect-resistant transgenic cotton has been produced by inserting a piece of DNA from?
  • An insect
  • A bacterium
  • A wild relative of cotton
  • A virus
Which of these is a true statement
  • Vectors carry only the foreign gene into the host cell
  • Plasmids can carry recombinant DNA but viruses cannot
  • Both plasmids and viruses can serve as vectors
  • All of these statements are correct
DNA polymerase enzyme is isolated from which bacteria?
  • E.Coli
  • Thermus aquaticus
  • Bacillus thrunegenesis
  • Agrobacterium
If the initial number of template DNA molecules in a PCR reaction is $$1000$$, the number of product DNA molecules at the end of $$20$$ cycles will be closest to.
  • $$10^3$$
  • $$10^6$$
  • $$10^9$$
  • $$10^{12}$$
Each restriction enzyme cleaves a molecule only at
  • methyl groups
  • the ends of genes
  • the time of DNA replication
  • a particular nucleotide sequence
E.coli which was grown in the medium containing heavy isotope $$ _{  }^{ 15 }{ N }$$ for several generations is shifted to normal $$_{  }^{ 14 }{ N }$$ medium, the percentage of DNA strands with $$_{  }^{ 14 }{ N }$$ isotope after two generations is ______________.
  • $$12.5$$%
  • $$50$$%
  • $$75$$%
  • $$100$$%
One strand of the given DNA segment codes for mRNA having the sequence AUC GCG UCA for the synthesis of protein. Which DNA strand is responsible for the above mRNA sequence ________________.
  • ATC-TAG

    GCC-GGC

    ATU-TAG
  • TAG-ATC

    CGC-GCG

    AGT-TCA
  • TGA-ACT

    CGC-GCG

    TAG-ATC
  • AGA-TCT

    GCG-CGC

    GAT-CTA
Which does not describe a function of the DNA polymerase molecule?
  • Recognise the free nucleotide that pairs with the base on the template strand of DNA
  • Read the strand of template DNA and recognise the base there
  • Proofread to ensure that the proper base has been incorporated
  • Make the proper nucleotide to match with the base read on the template strand
What is the function of the polymerase chain reaction in genetic engineering?
  • Cut DNA into many fragments
  • Carry DNA into a new cell
  • Link together newly joined fragments of DNA
  • Make millions of copies of a specific segment of DNA
A.......... is multiple, identical copies of a collection of DNA fragments inserted into plasmids
  • DNA clone
  • gene map
  • DNA probe
  • DNA library
Which of the following is not a technique for introducing recombinant DNA into host cells?
  • Infection by Agrobacterium
  • Microinjection
  • Electrophoresis
  • Infection by bacteriophage
What is the function of gel electrophoresis in genetic engineering?
  • Cut DNA into many fragments
  • Link together newly joined fragments of DNA
  • Make millions of copies of a specific segment of DNA
  • Separate fragments of DNA by their length and electrical charges
What is the function of a vector in genetic engineering
  • Cut DNA into many fragments
  • Carry DNA into a new cell
  • Link together newly joined fragments of DNA
  • Make millions of copies of a specific segment of DNA
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