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Ma 7 Quiz
are the short sections of DNA that are synthesized on the lagging strand of the replicating DNA.
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okazaki fragments
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DNA polymerase I
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RNA primase
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leading strand
The enzyme that can replicate DNA is called
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DNA polymerase
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DNA polymerase-DNA polymerase catalyzes the addition of nucleotides to a growing DNA chain.
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phosphodiester
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DNA polymerase I
During DNA replication, an open section of DNA, in which a DNA polymerase can replicate DNA, is called a
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replication fork
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RNA primase
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okazaki fragments
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leading strand
The new DNA strand that grows continuously in the 5' to 3' direction is called the
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DNA polymerase I
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replication fork
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okazaki fragments
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leading strand
Okazaki fragments appear on the lagging strand during DNA replication.
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lagging
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DNA polymerase
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DNA polymerase I
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discontinuous
DNA ligase then forms ___bonds between DNA Okazaki fragments.
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phosphodiester
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DNA polymerase I
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DNA polymerase
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discontinuous
What is the purpose of raising the temperature to 90-95°C at the beginning of each cycle of PCR?
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ligase-Ligase is not required for a PCR reaction. The enzyme used during PCR is a thermostable DNA polymerase.
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Single‑strand binding proteins stabilize the open conformation of the unwound DNA.
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To separate the double‑stranded DNA-The temperature is raised to denature the double‑stranded DNA molecule into single strands.
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the temperature is raised so that taq polymerase can extend the primers-The temperature is raised to 70-75∘C, the temperature over which taq polymerase is optimally active.
After replication is complete, the new DNAs, called _____, are identical to each other.
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RNA primase
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leading strand
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daughter DNA
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okazaki fragments
The data obtained from the Meselson-Stahl experiment after one generation of replication eliminated the dispersive model of DNA replication.
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the temperature is raised so that taq polymerase can extend the primers-The temperature is raised to 70-75∘C, the temperature over which taq polymerase is optimally active.
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False
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F-The data obtained from the Meselson-Stahl experiment after one generation was consistent with both the semiconservative and the dispersive model of DNA replication. The conservative model of DNA replication was eliminated because it predicted that there would be two bands representing the original DNA at one density and the newly replicated DNA at a different density.
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the 5' to 3' polarity restriction
If there are five molecules of DNA containing the target region at the beginning of a PCR reaction, how many copies of the target will be present after three rounds of amplification?
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RNA primase
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ligase-Ligase is not required for a PCR reaction. The enzyme used during PCR is a thermostable DNA polymerase.
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40-doubled after each replication cycle
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okazaki fragments
Which of the following molecules is not required for a PCR reaction?Ligase DNTPs DNA Primer
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ligase-Ligase is not required for a PCR reaction. The enzyme used during PCR is a thermostable DNA polymerase.
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Single‑strand binding proteins stabilize the open conformation of the unwound DNA.
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DNA polymerase-DNA polymerase catalyzes the addition of nucleotides to a growing DNA chain.
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To separate the double‑stranded DNA-The temperature is raised to denature the double‑stranded DNA molecule into single strands.
he role of the primers in PCR is _______.
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denaturation, annealing, and extension-The double-stranded product is denatured, the primers anneal to the target sequence, and then DNA synthesis (extension) by Taq polymerase proceeds.
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the temperature is raised so that taq polymerase can extend the primers-The temperature is raised to 70-75∘C, the temperature over which taq polymerase is optimally active.
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to define the target region and provide a 3' end that can be extended by taq polymerase-Primers bind to end of the target DNA strands, then taq polymerase synthesizes a new strand using the target DNA as a template.
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To separate the double‑stranded DNA-The temperature is raised to denature the double‑stranded DNA molecule into single strands.
RNA primers are formed by ____ and serve as an initiation point for DNA synthesis on the template.
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DNA polymerase
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okazaki fragments
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RNA primase
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DNA polymerase I
Okazaki fragments are short DNA fragments synthesized in a _____manner.
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phosphodiester
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DNA polymerase I
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discontinuous
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lagging
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