A gene, whose expression helps to identify transformed cells is known as

  • selectable marker                 

  • vector

  • plasmid                                 

  • structural gene

The DNA fragments separated on an agarose gel can be visualised after staining with

  • bromophenol blue

  • acetocarmine

  • aniline blue 

  • ethidium bromide

Stirred-tank bioreactors have been designed for:

  • purification of product

  • addition of preservatives to the product

  • availability of oxygen throughout the process

  • ensuring anaerobic conditions in the culture vessel

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

  • Eco RI                       

  • taq polymerase

  • polymerase III           

  • ligase

Which of the following is not a component of downstream processing?

  • Separation

  • Purification

  • Preservation

  • Expression

Which of the following restriction enzymes produces blunt ends?

 

  • Sal I                           

  • Eco RV

  • Xho                           

  • Hind III

Which of the following is a endonuclease?

  • Protease

  • DNase I

  • RNase restriction

  • Hind II

The Taq polymerase enzyme is obtained from

  • Thiobacillus ferroxidans

  • Bacillus subtilis

  • Pseudomonas subtilis

  • Thermus aquaticus

The cutting of DNA at specific locations became possible with the discovery of 

  • restriction enzymes

  • probes

  • selectable markets

  • ligases

The DNA molecule to which the gene of interest is integrated for cloning is called

  • Transformer

  • Vector

  • Template

  • Carrier 

Which vector can clone only a small fragment of DNA?

  • Bacterial artificial chromosome

  • Yeast artificial chromosome

  • Plasmid

  • Cosmid

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

  • centrifugation

  • polymerase chain reaction

  • electrophoresis

  • restriction mapping

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

  • Bacteria —Lysozyme

  • Plant cells — Cellulase

  • Algae —Methylase

  • Fungi — Chitinase

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

 

  • Non-recombinant bacteria containing β-galactosidase

  • Insertional inactivation of α-galactosidase in non-recombinant bacteria

  • Insertional inactivation of β-galactosidase in recombinant bacteria

  • Inactivation of glycosidase enzyme in recombinant bacteria

The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

  • Ori-original restriction enzyme

  • rop-reduced osmotic pressure

  • Hind III, Eco RI -selectable markers

  • ampR, tetR-antibiotic resistance genes

Which one of the following is a case of wrong matching ?

  • Somatic hybridisation - Fusion of two diverse cells

  • Vector DNA - Site for tRNA synthesis

  • Micropropogation - In vitro production of plants in large numbers

  • Callus - Unorganised mass of cells produced in tissue culture

Which one is a true statement regarding DNA polymerase used in PCR?

  • It is used to ligate introduced DNA in recipient cells

  • It serves as a selectable marker

  • It is isolated from a virus

  • It remains active at high temperature

For transformation, microparticles coated with DNA to be bombarded with gene gun are

made up of

  • silver or platinum

  • platinum or zinc

  • silicon or platinum

  • gold or tungsten

Given below is a sample of portion of DNA strand giving the base sequence on the

opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

  • Deletion mutation

  • Start codon at the 5' end

  • Palindromic sequence of base pairs

  • Replication completed

There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

  • Coelom                         

  • Coenzyme

  • coli                               

  • Colon

Agarose extracted from sea weeds finds use in

  • tissue culture

  • PCR

  • gel electrophoresis

  • spectrophotometry

Continuous addition of sugars in ‘fed batch’ fermentation is done to

  • obtain antibiotics

  • purify enzymes

  • degrade sewage

  • produce methane

Which one of the following is used as vector for cloning genes into higher organisms?

  • Baculovirus

  • Salmonella typhimurium

  • Rhizopus nigricans

  • Retrovirus

Which one of the following palindromic base sequences in DNA can be easily cut at about

the middle by some particular restriction enzyme?

     3'-ATGGTA--5'

     3'-CTACTA-5'

     3'-CTTAAG-5'

     3'-CTCAGT-5'

  • 5'-CGTTCG-3'

  • 5'-GATATG-3'

  • 5'-GAATTC-3'

  • 5'-CACGTA-3'

Restriction endonucleases are enzymes which

  • make cuts at specific positions within the DNA molecule

  • recognize a specific nucleotide sequence for binding of DNA ligase

  • restrict the action of the enzyme DNA polymerase

  • remove nucleotides from the ends of the DNA molecule

Stirred-tank bioreactors have been designed for

  • addition of preservatives of the product

  • purification of the product

  • ensuring anaerobic conditions in the culture vessel

  • availability of oxygen throughout the process

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  •  Trichoderma harzianum

  •  Meloidogyne incognita

  •  Agrobacterium tumefaciens

  •  Penicillium expansum

Polyethylene glycol method is used for

  • gene transfer without a vector

  • biodiesel production

  • seedless fruit production

  • energy production from sewage

Gel electrophoresis is used for

  • cutting of DNA into fragments

  • separation of DNA fragments according to their size

  • construction of recombinant DNA by joining with cloning vectors

  • isolation of DNA molecule

The linking of antibiotic resistance gene with the plasmid vector became possible with

  • DNA ligase               

  • endonucleases

  • DNA polymerase       

  • exonucleases

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