Microbes found to be very useful in genetic engineering are:

  • Escherichia coli and Agrobacterium tumefaciens

  • Vibrio cholerae and a tailed bacteriophage

  • Diplococcus sp. and Pseudomonas sp.

  • Crown gall bacterium and Caenorhabditis elegans

Restriction endonuclease :

  • cuts the DNA molecule-randomly

  • cuts the DNA molecule at specific sites

  • restricts the synthesis of DNA inside the nucleus,

  • synthesizes DNA

Which of these are most widely used in genetic engineering?

  • Plastid

  • Plasmid

  • Mitochondrion

  • Ribosome

Which of the following is a cloning vector?

  • DNA of Salmonella typhimurium

  • Ti plasmid

  • Amp' and Tet' loci

  • Ori minus PBR322

Which of the following is a palindromic sequence?

      3'-CGAATG-5'

     3'-GCATAC-5'                 

     3'-CTTAAG-5'      

     3'-CTTAAG-5'      

  • 5'-CGTATG-3'           

  • 5'-CGAATG-3'

  • 5'-GAATTC-3'

  • 5'-GACTAC-3'

How are transformants selected from nontransformants?

  • Presence of more than one recognition site in the vector DNA.

  • Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker.

  • Antibiotic resistance gene gets inactivated due to insertion of alien DNA.

  • Both 2 and 3.

Which of the following is not a characteristic of pBR322 vector? 

  • It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.

  • It is the most widely used, versatile and easily manipulated vector.

  • It has two antibiotic resistance genes, tetR and ampR.

  • It does not have restriction site for Sal I.

Select the correct order of processing of PCR.

  • Extension, primer annealing, denaturation

  • Denaturation, primer annealing, extension

  • Denaturation, extension, primer annealing

  • Primer annealing, denaturation, extension

Plasmid vector in DNA recombinant technology means

  • a virus that transfers gene to bacteria

  • extra-chromosomal autonomously replicating circular DNA

  • sticky end of DNA

  • any fragment of DNA carrying desirable gene

Which organism is used to transfer T-DNA?

  • Streplomyces hygroscopicus

  • Agrobacterium tumefaciens

  • Salmonella typhi

  • Escherichia coli

Match the following column I with column II.

Column I                                      Column II

A. Agrostology                              1. Earth

B. Smallest flowering plant            2. Genetic engineering

C. Tectonic                                   3.Migration

D. Ecesis                                      4.Wolffia

E. Euphanix                                  5.Grass

Codes

       A     B     C     D     E

  • (c)   2     3     1     5     4

  • (d)   5     4     3     2     1

  • (b)   1     2     3     4     5

  • (a)   5     4     1     3     2   

Biolistic (gene gun) is suitable for

  • disarming pathogen vectors

  • transformation of plant cell

  • constructing recombinant DNA by joining with vectors

  • DNA fingerprinting

A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

  • b, a, c, d

  • a, b, c, d

  • c, b, a, d

  • b, a, d, c

Which of the following DNA sequences qualifies to be designated as a palindrome?

  • 5 - GACCAG - 3’ in one strand

  • 3’- GACCAG - 5’ in one strand

  • 5’- GACGAG- 3’ 3’- CTGGTC - 5’

  • 5’- AGCGCT - 3’ 3’- TCGCGA - 5’

The source of taq polymerase used in PCR is a

  • thermophilic fungus

  • mesophilic fungus

  • thermophilic bacterium

  • halophilic bacterium

pBR322, which is frequently used as a vector for cloning gene in E. coli is a/an

  • original bacterial plasmid

  • modified bacterial plasmid

  • viral genome

  • transposon

Which of the following statement is not true: 

  • Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

  • Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli

  •   The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin

  • Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning

Go through the statements:

A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinsulin.

B. In hydridoma technology, B-cells are fused with myeloma cells.

C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccine before use in humans.

D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.

E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.

F. Gray biotechnology' is referred to industrial process.

G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA. 

How many statement(s) is wrong?

  • One

  • Two

  • Four

  • Six

Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be:

A. Denatured double stranded DNA probes

B. doubles stranded RNA probes

C. Protein probes

D. Single stranded DNA probes.

  • A, B

  • B, C

  • A, D

  • A, B, C, D

Which is a natural genetic engineer of the plants?

  • E.coli

  • A. tumefaciens

  • Rhizobium

  • Pseudomonas

The thermostable enzyme Taq polymerase used in PCR technique was discovered by

  • Alec Jeffrey

  • Karry Mullis

  • Temin

  • Baltimore

To be useful in genetic engineering a vector must have

    1. An origin for replication
    2. Low copy number
    3. Low rates of multiplication
    4. Many cloning sites for the same enzyme
  • 1
  • 2
  • 3
  • 4

In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was

  • Methylase

  • Exonuclease

  • Aminotransferase

  • Terminal Transferase

 E.coli is  a commonly used host for gene cloning because

  • It is free from elements that interfere with replication and recombination of DNA

  • It is easy to transform

  • It supports replication of inserted DNA.

  • All of these

A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

  • .  Suppression vector

  • . Expression vector

  • . Cloning vector

  • . None of these

Electroporation facilitates insertion of foreign DNA into the cell by

  • Changing the porocity of the membrane

  • Changing the electrical potential of the membrane

  • Lysing the cell wall

  • Active transport

The names associated with construction of first Recombinant DNA are

  • . Arber, Nathans, Smith

  • . Annie Chang, Boyer, Berg, Cohen

  • . Howard Temin, Brenner, Sharp

  • . Tim Hunt, Hartwell, Nurse

The restriction enzymes most commonly used in rDNA technology are

  • Type 1 Restriction enzymes

  • Type II Restriction enzymes

  • Type III Restriction enzymes

  • Type IV Restriction enzymes

Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because

  • . Host DNA is packed into chromosomes
  • . Restriction enzymes are ineffective on host DNA
  • . Host DNA does not have the restriction site for the Restriction enzymes.
  • . Restriction site of host DNA is methylated.

A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?

  • . Southern blotting and South Western blotting
  • . Northern blotting and western blotting
  • . Western blotting and Eastern blotting
  • . Southern and Northern blotting

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