Insertional inactivation of a gene helps in

  • . Identification of recombinant clones
  • . Identification of deletion mutants
  • . Identification of recombinant transformants
  • . Elimination of suppression mutants

Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of

  • . Zinc or tungsten
  • . Silicon or gold
  • . Tungsten or gold
  • . Selenium or Platinum

Insertional inactivation of the lac Z gene forms-

  • Blue recombinant colonies

  • Colourless recombinant colonies

  • Fluorescent green colonies

  • There is no relation between the lac Z gene and colour of the colony.

A reporter enzyme used in biotechnology is-

  • Green Fluorescent Protein

  • Luciferase

  • b Galactosidase

  • All of the above

Downstream processing does not include

  • Quality control

  • Clinical trials

  • Product purification

  • Sampling from bioreactor

Which is not a component of the vector pBR322?

  • Chloramphenicol selectable marker

  • Replication proteins

  • Origin of replication

  • Cloning site for BamHI enzyme

Which among the following is a cloning vector?

  • EcoRI

  • BamHI

  • pUC18

  • Sal I

Two bacteria found to be very useful in genetic engineering are

  • Diplococcus and Streptomyces

  • Rhizobium and Xanthomonas

  • Vibrio and Escherischia

  • Escherischia and Agrobacterium

Which of the following would you choose as the safest and least cumbersome selectable marker-

  • Ampicillin resistance gene

  • Tetracyclin resistance gene

  • Kanamycin resistance gene

  • b Galactosidase gene

Recombinant DNA is formed in nature during-

  • Crossing over at pachytene

  • Integration of plasmid to form episome

  • Integration of phage DNA to form prophage

  • More than one is correct.

Southern Blotting essentially makes use of a

  • Buffer

  • Electrodes

  • Agarose gel

  • All of the above

The native charge on DNA molecules is

  • Positive

  • Negative

  • Neutral

  • DNA is an ampholyte

The visualization of the DNA bands on the gel can be done with the help of

  • UV rays

  • Ethidium bromide

  • Chromogenic substrate

  • More than one is correct

If one needs to derive a cDNA of human insulin, which cells should be taken up for extraction?

  • Any cell of the human body as the DNA is same in all cells

  • Any nucleated cell of the human body

  • b cells of islets of Langerhans of human pancreas

  • WBCs

The natural genetic engineer of plants is

  • Armillaria ostoyae

  • Agrobacterium tumefacians

  • Argemonaria geneticis

  • Nuclearia incorporate

A distinct advantage of continuous culture over batch culture is

  • Higher productivity

  • Versitality

  • Can be used for different reactions everyday

  • Continuous inflow of nutrients

Plasmids are good vectors for genetic engineering because

  • They self replicate within bacterial cells

  • Replicate freely outside bacterial cells

  • Can be replicated in culture

  • Can be replicated in laboratory using enzymes

Molecular weight of DNA in diploid human cell in Da is

  1. 3.6 X 10 12 Da
  2. 46 X 10 10
  3. 30 billion Da
  4. 46 billion Da
  • 1
  • 2
  • 3
  • 4

The Ti in Ti plasmid stands for

  • Tumour inciting

  • Tumour inducing

  • Transposon incumbent

  • Transition impact

Classical biotechnology does not include the production of

  • Curd

  • Ethyl alcohol

  • Insulin from bacteria

  • Citric acid

Why is a marker gene useful?

  • It shows you whether the gene being added, has been taken up or not.

  • It's a way of labelling which gene you want to modify.

  • It sticks with the striker gene.

  •   It marks the position of r-DNA

Transformation is the term used to denote

  • Non virus mediated gene transfer in non Animal eukaryotic cells

  • Virus mediated gene transfer in Bacteria

  • Virus mediated gene transfer in non Animal eukaryotic cells

  • All of the above

3. In a type of yeast fermentation , the entire medium with fermenting organism is removed from the bioreactor after reaching an alcohol level of 10-12%. This process is called

  • . Continuous process
  • . Batch process
  • . Synchronous culture
  • . Immobilized process

The introduction of genes into plant cells often makes use of

  • . Disarmed Agrobacterium vectors
  • . disarmed retroviral vectors
  • . Ti plasmid of Agrobacterium
  • c. Ri plasmi of Agrobacterium

According to the traditional view which of the following can be kept under the process or form in Biotechnology?

  • Test tube baby

  • DNA vaccine

  • Making of wine

  • Correcting a gene

The definition of biotechnology given by EFB encompasses

  •       Traditional view

  •       Modern molecular biotechnology

  •       Both 1 and 2

  •      Only Molecular analogs

Which of the following is not a core technique which gave birth to modern biotechnology?

  •       Genetic engineering

  •       Bioprocess engineering

  •       Fermentation

  •      Both 1 and 2

Which of the following products do not need bioprocess engineering?

  •       Antibiotics

  •       Vaccines

  •       Enzymes

  •      Curd

Variations which are the results of sexual reproduction will

  •       Always bad for a population

  •       Not always good for individual but for population

  •       Not always good for individual and population

  •      Always good for individual

Traditional hybridization procedures lead to the inclusion and multiplication of undesirable genes along with desirable genes. Which of the following processes cannot help to rid this undesirable Gene?

  •       creation of recombinant DNA

  •       Use of gene cloning

  •       Use of gene transfer

  •      Use of Agarose gels

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