If you transform a cell with an alien piece of DNA only, What is the possibility?

  •       This alien piece becomes a part of the host genome.

  •       It doesn't become a part of the host's genome but keeps on replicating

  •       The cloning of genes won't get affected in the absence of Origin of replication.

  •      Multiple identical copies of an alien piece of DNA can be formed irrespective of its integration into the host's genome.

Which of the following is not associated with first recombinant DNA molecule?

  •       Plasmid of Salmonella typhimurium

  •       Antibiotic resistance gene

  •       1975

  •      Stanley Cohen

If you want to isolate a particular gene from a plasmid or genome of an organism which of the following enzyme has to be used?

  •       DNA ligase

  •       Lysozyme

  •       Restriction enzyme

  •      DNA polymerase

Cohen and Boyer are known for which of the following discoveries?

  •       Antibiotic resistance gene

  •       Recombinant DNA

  •       Restriction Digestion

  •      Plasmid

Arrange the following enzymes/ processes in the order of their use for a certain experiment of genetic engineering:

1) ligase,
2) restriction enzyme
3)desired gene
4) Transformation
5) Expression

  •       3-2-1-4-5

  •       1-2-4-5-3

  •       3-1-4-5-2

  •      2-5-4-3-1

What does cloning mean?

  •       The ability to multiply the desired gene in the host

  •       The ability to multiply the vector in the host

  •       The ability to multiply the antibiotic resistance gene in the host

  •      The ability to multiple Ori in the host

Which of the following is not a tool of RDT?

  •       PCR

  •       Restriction enzymes

  •       Vectors

  •      Host organisms

Which of the following is a tool of RDT?

  •       PCR

  •       Gel Electrophoresis

  •       Cloning

  •      Restriction enzyme

In the year 1963, the two enzymes responsible for inhibiting the growth of bacteriophage in E. coli were isolated. One of them was

  •       Ligase

  •       Polymerase

  •       Molecular scissor

  •      Antibiotic resistance enzyme

Hind II

  •       First restriction endonuclease

  •       Was characterized 7 years later

  •       Specific sequence of 5 base pairs

  •      All of these

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

  •       700; 350

  •       900; 230

  •       600; 270

  •      550; 210

Which of the following is correct representation of the name of enzyme?

  •       ECORI

  •       EcoRI

  •       ECoRI

  •      ECoR1

EcoRI comes from

  •       Escherichia coli RY13

  •       Escherichia coli RY16

  •       Escherichia coli RY19

  •      Escherichia coli RY12

In EcoRI, here I stands for

  •       Order in which strains are discovered

  •       Order in which enzymes are isolated

  •       Order in which enzymes are isolated from that strain

  •       Order in which strains are reported in microbiology

Restriction endonuclease

  •       Directly recognizes a specific sequence

  •       Makes the cut at the sugar-phosphate core.

  •       Comes under nuclease

  •       Both 1 and 3

Restriction endonuclease cuts

  • At palindromic sequences for some enzymes not for all

  • After scanning and recognition of specific sequence in a DNA strand

  • Cuts the strand exactly at the centre

  • All of these

Restriction enzyme EcoRI cuts

  •       Between bases G and A

  •       Cuts between GAATTC

  •       Cuts between G and A  at GAATTC

  •       Both 1 and 3

EcoRI cuts

  •       A little away from the centre

  •       Both the strands at the same time

  •       Creates blunt ends

  •       Both 1 and 2

DNA gel electrophoresis is commonly prepared by

  •       Agarose, a natural polymer

  •       Extracted from seaweeds

  •       A synthetic polymer

  •       Both 1 and 2

Which of the following step is first one in order to separate DNA fragments?

  •       Running of gel

  •       Staining of gel

  •       Elution

  •      Setting of gel

Which of the following step is last for isolating the desired DNA fragment?

  •       Running of gel

  •       Elution

  •       Staining

  •      Visualization

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

  •       1-2-3-4-5-6-7

  •       1-5-6-7-2-3-4

  •       1-2-3-5-6-7-4

  •       2-3-4-1-5-6-7

Which of the following steps will be performed after restriction digestion?

  •       Ligation

  •       Elution from DNA gel

  •       Transformation

  •       Cloning

If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

  •       a- bacteriophage

  •       a- Plasmid

  •       a- bacteriophage or Plasmid

  •      a- prion

Which of the following is not a feature to facilitate cloning into a vector?

  •       Origin of replication

  •       Selectable Marker

  •       Multiple cloning site

  •       Virulence region

If I want to check the expression of my gene, then I should use

  •       Vectors supporting high copy number

  •       Vectors supporting low copy number

  •       No need to bother about copy number

  •      Vectors with strong Ori

Selectable marker helps

  •       To identify transformants

  •       To identify recombinants

  •       To identify non- recombinants

  •       To eliminate transformants

The normal E. coli cells have

  •       Kanamycin

  •       Tetracycline

  •       Ampicillin

  •      No resistance

For easiest cloning, one needs to get vector with

  •       Single cloning site

  •       Multiple cloning site

  •       Few cloning site

  •      No cloning site at all

If the gene of Beta-galactosidase is not inactivated, means there will be color in the colonies of

  •       Non- recombinant

  •       Recombinant

  •       Transformants

  •      Non-transformants

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