Insertional inactivation results in

  •       Non-recombinants

  •       Recombinants

  •       Transformants

  •      Non- transformants

Which of the following options is correct about Agrobacterium tumifaciens?

  •      It is a pathogen of angiosperms

  •      It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

  •      Its Ti plasmid is now modified into an expression vector

  •      Its Ti plasmid is modified into a cloning vector which is no more pathogenic

In order to make the host cell competent

Divalent cations such as calcium are used-

  •       The divalent ions have to be in a specific concentration

  •       They cause the DNA uptake by the cell

  •       They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

  •      All of the above

The process of competent cell formation include

  •       CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

  •       Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

  •       CaCl2 treatment - Heat shock -Ice incubation with r-DNA

  •      Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

Micro-injection is a method of introducing alien DNA into

  •       Cytoplasm of all cells

  •       Cytoplasm of bacterial cell

  •       Nucleus of animal cell

  •      Nucleus of plant cell

Biolistics

  •       Plant cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA

  •       Also known as gene gun

  •       Both A and B

  •      Direct injection

pBR322 means

  •       BR for the initials of two scientists who constructed it

  •       BR for the resistance of BamH1

  •       BR for the multiple cloning site

  •      BR  for its discovery in Berlin

The antibiotic resistance gene in pBR322 is not

  •       Tetracycline

  •       Chloramphenicol

  •       Ampicillin

  •      Both A and B

rop segment codes for pBR322?

  •       Resistance protein

  •       Protein for replication

  •       Proteins for translation

  •      Cloning site

Pvu II restriction site is present in pBR322 at?

  •       rop

  •       Tetracycline resistance gene

  •       Ampicillin resistance gene

  •      Ori

BamHI restriction site is present in pBR322 at?

  •       rop

  •       Tetracycline resistance gene

  •       Ampicillin resistance gene

  •      Ori

Sal I restriction site is present in pBR322 at?

  •       rop

  •       Tetracycline resistance gene

  •       Ampicillin resistance gene

  •      Ori

Pvu I restriction site is present in pBR322 at?

  •       rop

  •       Tetracycline Resistance gene

  •       Ampicillin Resistance gene

  •      Ori

If you have a fungal cell and want to extract DNA, what will be the first enzyme to be used?

  •       Chitinase

  •       Lysozyme

  •       Cellulase

  •      Protease

If a researcher starts his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

  •       Cellulase

  •       Protease

  •       Ribonuclease

  •      Chitinase

If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

  •       Cellulase

  •       Chitinase

  •       Lysozyme

  •      Protease

If a researcher has to use a bacterial cell to isolate DNA from bacterial cells then to remove another nucleic acid, which enzyme should be used?

  •       DNAse

  •       Ribonuclease

  •       Protease

  •       Lysozyme

The precipitation of DNA out of the solution is done by adding

  •       Organic Acid

  •       HCl

  •       Ethanol

  •      Ketone

The end result of precipitation of DNA in suspension is seen

  •       In the form of dark solution

  •       In the form of effervescence

  •       In the form of fine threads

  •       In the form of bubbles

The precipitated DNA can be removed from the suspension

  •       By Filtration

  •       By giving a heat shock

  •       By spooling

  •       By gel electrophoresis

Primers are not

  •       Small

  •       Biologically synthesized

  •       Oligonucleotides

  •      Complementary to the regions of DNA

Which of the following doesn't go with PCR?

  •      Thermus aquaticus

  •      1 billion copies

  •      Taq polymerase

  •      Dideoxynucleotides

If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

  •       5’TAACGGGATC 3’

  •       3’TAACGGGATC 5’

  •       5’ATTGCCCTAG 3’

  •      3’ATTGCCCTAG 5’

If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

  •       10240

  •       1024

  •       512

  •      5120

What is the final step of PCR?

  •       Extension

  •       Annealing

  •       Denaturation

  •      Extraction

After 30 cycles of PCR, how many times DNA will be amplified?

  •       Thousand times

  •       Million times

  •       Billion times

  •      Hundreds times

Use of primers, occurs in which step of PCR?

  •      Annealing

  •      Extension

  •      Denaturation

  •      Both extension and Annealing

The ultimate aim of maximum recombinant DNA technologies is

  •    To multiply DNA

  •    To express DNA

  •    To multiply mRNA

  •    To express protein

Which of the following processes is not associated with the protein produced by recombinant DNA?

  •       Restriction Digestion

  •       Expression

  •       Optimization

  •      Purification

What does continuous culture mean?

  • Where DNA and protein getting expressed continuously

  • Where used medium is drained out and the fresh medium is added from another side

  • Where cells are producing protein for one week continuously

  • Where the production of recombinant DNA is continuing without interference.

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