Continuous culture has

  •       Cells in their physically active phase

  •       Lag phase

  •       Both A and B

  •      Exponential phase

Continuous culture leads to

  • Smaller biomass

  • Higher yields of desired proteins

  • Both 1 and 2

  • Higher yields of recombinant DNA

The volume of bioreactors cannot be

  •       20 L

  •       100L

  •       150L

  •      200L

Bioreactors don't provide which of the conditions?

  • Salts

  • Vitamins

  • Cells

  • Oxygen

The most commonly used bioreactors are

  • Stirring type

  • Sparged type

  • Aerobic type

  • Both 2 and 3

The cylindrical body or curved base of a bioreactor actually facilitates

  • The handling and maintenance of bioreactor

  • mixing

  • better oxygen transport

  • more accumulation of product

Increased surface area for oxygen transfer is a feature found specifically in

 

1.      Simple stirred tank bioreactor

 

2.      Sparged stirred-tank bioreactor

 

3.      All stirred tank bioreactors

 

4.     All bioreactors have this property
  • 1
  • 2
  • 3
  • 4

Downstream processing includes

 

 

 

 

  •       Separation

  •       Purification

  •       Both 1 and 2

  •      Quality control Testing

The Downstream processing occurs after

 

1.      Biosynthetic phase

 

2.      Separation phase

 

3.      Purification phase

 

4.     All of these
  • 1
  • 2
  • 3
  • 4

Quality control testing is not

 

 

 

 

  •       A part of downstream processing

  •       Include separation and purification

  •       Both 1 and 2

  •      Vary from product to product

Downstream processing is not

 

  •       Separation

  •       Purification

  •       Vary with product

  •      Occurs during the marketing phase

The formulation with suitable preservatives in the case of drugs has to undergo

 

  •       Through clinical trials

  •       Through strict separation techniques

  •       Through harsh heat shock control

  •      Through long restriction digestion

Which of the following is the reason for the increased surface area for oxygen transfer in a sparged bioreactor ?

  • Bubbles

  • Turbidity

  • Cells

  • Protein

If a sample has a DNA of size 2kb and 6kb while the molecular marker denotes 4kb band as its the first band where will be the 6kb band of DNA?

  • bove the band of another DNA sample

  • Above the band of marker

  • In between the band of the marker and another band of DNA sample.

  • Both A and B

An important limitation to the use of Agrobacterium tumefaciens is that it can not:

  • infect dicots

  • be genetically modified

  • be cultured on a nutrient medium

  • infect crop plants such as wheat and corn

The specific palindromic sequence which is recognized by EcoRI is :

    3'-CCTTGG-5'

    3'-GAATTC-5'

    3'-CCTAGG-5'

    3'-CTTAAG-5'

  • 5' -GGAACC-3'

  • 5'-CTTAAG-3'

  • 5'-GGATCC-3'

  • 5'-GAATTC-3'

Match each item in Column I with one item in Column II and chose your answer from the codes given below:
Column I                             Column II
(restriction enzyme)            (recognition site)
I. EcoRI                              1. GAATTC
II. SmaI                              2. CCCGGG
III. ScaI                              3. AGTACT
IV. BamHI                            4.GGATCC
Codes:
     I    II    III   IV
  • .  1    2     3    4
  • .  2    1    4    3
  • .  2    1    3    4
  • .  1    2    4    3

What is true about the steps of PCR shown in the diagram given below?

I. Step A occurs at the temperature of 95 – 980 C
II. Step B occurs at the temperature of 550 C
III. Step C occurs at the temperature of 720 C

  • I and II only
  • I and III only
  • II and III only
  • I, II and III

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

  • southern blotting

  • northern blotting
  • polymerase chain reaction
  • DNA replication

Agrobacterium tumefaciens is

  • a disease in humans that causes loss of sight

  • a bacterium that can be used to introduce DNA into plants
  • a fungi that is used to produce antibiotics in large amounts
  • a disease in humans that causes loss of weight

Vectors are

  • molecules that degrade nucleic acids

  • molecules that help in replication
  • molecules that are able to covalently bond to and carry foreign DNA into cells
  • molecules that protect host cells from invasion by foreign DNA

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

  • DNA polymerase

  • Restriction endonuclease
  • DNA ligase
  • DNA helicase

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

  • laser

  • DNA probe

  • gene gun

  • inoculating needle

Bacterial resistance to antibiotics is a genetic trait carried in the bacterial:

  • Intron

  • Chromosome

  • Plasmid

  • Centromere

The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation
  • Denaturation, Extension, Annealing
  • Denaturation, Annealing, Extension

DNA fragments are:

  • Negatively charged

  • Neutral

  • Either positively charged depending on their size

  • Positively charged

Which of the following is a restriction endonuclease?
  • Protease
  • DNase I
  • RNase
  • Hind II
Which of the following is not a feature of the plasmids ?
  • Circular structure
  • Transferable
  • Single-stranded
  • Independent replication

The introduction of t-DNA into plants involves

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
0:0:1


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Practice Zoology Quiz Questions and Answers