Which one is a true statement regarding DNA polymerase used in PCR?
It is used to ligate introduces DNA in recipient cells
It serves as a selectable marker
It is isolated from a virus
It remains active at high temperature
tissue culture
PCR
gel electrophoresis
spectrophotometry
Restriction endonucleases are enzymes which
make cuts at specific positions within the DNA molecule
recognize a specific nucleotide sequence for binding of DNA ligase
restrict the action of the enzyme DNA polymerase
remove nucleotides from the ends of the DNA molecule
In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with
corn
rice
wheat
soy beans
In the screening process, clones that metabolize X-gal turn
yellow
orange
blue
colorless
Restriction endonuclease:
cuts the DNA molecule randomly
cuts the DNA molecule at specific sites
restricts the synthesis of DNA inside the nucleus
synthesis DNA
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
The enzyme cuts DNA molecules at identified position within the DNA
The enzyme binds DNA at specific sites and cuts only one of the two strands
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
Chilled chloroform
isopropanal
Chilled ethanol
Methanol at room temperature
Which of the following equipments is essentially required for growing microbes on a large scale for industrial production of enzymes?
Bioreactor
BOD incubator
Sludge digester
Industrial oven
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
HindII
SmaI
BamHI
The following palindrome is recognized by the restriction enzyme:
EcoRI
PstI
Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
Bacteriophages
BAC
Plasmid
The DNA fragments separated on an agarose gel can be visualised after staining with
Match the following enzymes with their functions:
Column-I
Column-II
(a)
Restriction
endonuclease
(i)
joins the DNA fragments
(b)
Exonuclease
(ii)
extends primers on genomic DNA template
(c)
DNA ligase
(iii)
cuts DNA at specific position
(d)
Tag polymerase
(iv)
removes nucleotides from the ends of DNA
Select the correct option from the following:
(a) - (iii), (b)- (i), (c)- (iv), (d)-(ii)
(a) - (iii), (b)- (iv), (c)- (i), (d)-(ii)
(a) - (iv), (b)- (iii), (c)- (i), (d)-(ii)
(a) - (ii), (b)- (iv), (c)- (i), (d)-(iii)
The two antibiotic resistance genes on vector pBR 322 are for
Ampicillin and Tetracycline
Ampicillin and Chloramphenicol
Chloramphenicol and Tetracycline
Tetracycline and Kanamycin
A selectable marker is used to :
help in eliminating the non transformants so that the transformants can be regenerated
identify the gene for a desired trait in an alien organism
select a suitable vector for transformation in a specific crop
mark a gene on a chromosome for isolation using restriction enzyme
Given below are four statements pertaining to separation of DNA fragments using Gel electrophoresis. Identify the incorrect statements.
a. DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal.
b. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
c. Smaller the size of DNA fragment, larger is the distance it travels through it.
d. Pure DNA can be visualized directly by exposing to UV radiation.
Choose correct answer from the options given below:
(a), (c) and (d)
(a), (b) and (c)
(b), (c) and (d)
(a), (b) and (d)
An enzyme catalysing the removal of nucleotides from ends of DNA is :
Endonuclease
Protease
The sequence that controls the copy number of the linked DNA in the vector, is termed :
Ori site
Palindromic sequence
Recognition site
Selectable marker
In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
Ethidium bromide in UV radiation
Acetocarmine in UV radiation
Ethidium bromide in infrared radiation
Acetocarmine in bright blue light
Identify the wrong statement with regards to restriction enzyme
They cut the strength at palindromic sites.
They are useful in genetic engineering.
Sticky ends can be joined by using DNA ligases.
Each restriction enzyme functions by inspecting the length of a DNA sequence.
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognizing a specific sequence of six base pairs is:
EcoR1
Adenosine deaminase
Thermostable DNA polymerase
Hind II
In recombinant DNA technology antibiotics are used :
to keep medium bacteria-free
to detect alien DNA
to impart disease-resistance to the host plant
as selectable markers
Match the following techniques or instruments with their usage :
(a) Bioreactor (i) Separation of DNA fragments
(b) Electrophoresis (ii) Production of large quantities of products
(c) PCR (iii) Detection of pathogen, based on antigen - antibody reaction
(d) ELISA (iv) Amplification of nucleic acids
Select the correct option from following:
(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
(a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
(a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
(a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)
In a mixture, DNA fragments are separated by-
Bioprocess engineering
Restriction digestion
Electrophoresis
Polymerase chain reaction
Spooling is :-
Amplification of DNA
Cutting of separated DNA bands from the agarose gel
Transfer of separated DNA fragments to synthetic membranes
Collection of isolated DNA
Select the correct statement from the following :
Gel electrophoresis is used for the amplification of a DNA segment.
The polymerase enzyme joins the gene of interest and the vector DNA.
Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
PCR is used for isolation and separation of genes of interest.
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
A - Denaturation at a temperature of about 50ºC
C - Extension in the presence of heat stable DNA polymerase
A - Annealing with two sets of primers
B - Denaturation at a temperature of about 98ºC separating the two DNA strands
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:
Dark red bands
Bright blue bands
Yellow bands
Bright orange bands
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Extension, Denaturation, Annealing
Annealing, Denaturation, Extension
Denaturation, Annealing, Extension
Denaturation, Extension, Annealing
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