During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

  • Histones

  • Polysaccharides

  • RNA

  • DNA

Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

  • it will lead to the lysis of host cells.

  • it will be able to produce a novel protein with dual abilities. 

  •  it will not be able to confer ampicillin resistance to the host cell. 

  • the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is: 

  • Palindromic Nucleotide sequences

  • Poly(A) tail sequences

  • Degenerate primer sequence

  • Okazaki sequences

During the process of gene amplification using PCR, very high temperature is not maintained in the beginning, then which of the following PCR will be affected first? 

  • Denaturation

  • Ligation

  • Annealing

  • Extension

What is incorrect regarding DNA replication?
  • For a cell to divide, it must first replicate its DNA.
  • DNA replication is an all-or-none process; once replication begins, it proceeds to completion.
  • DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell).
  • Once replication is complete, it does not occur again in the same cell cycle.
Agrobacterium tumefaciens infects the plant through its:
 
  • Chromosomal DNA
  • Complementary DNA
  • F plasmid
  • Ti plasmid
To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Column I Column II
A Lysozyme P Fungal cell
B Cellulase Q Plant cell
C Chitinase R Bacterial cell

Codes
A B C
1. P Q R
2. P R Q
3. R P Q
4. R Q P
  • 1
  • 2
  • 3
  • 4
For their work in the discovery and characterization of restriction enzymes, the 1978 Nobel Prize for Physiology or Medicine was awarded to:
 
  •     Salvador Luria, Jean Weigle and Giuseppe Bertani
  •     Nirenberg, Matthaei and Korana 
  •     Paul Berg, Stanley Cohen and Herbert Boyer
  •     Werner Arber, Daniel Nathans, and Hamilton O. Smith
Beginning with a single template DNA, a PCR reaction is allowed to continue for 30 cycles. The number of copies of the template DNA at the end is expected to be about:
 
  •     256
  •     256,000
  •     1 million
  •     1 billion
PCR does not require:
 
  •     A thermostable DNA polymerase
  •     Dideoxy-dNTPs (ddNTPs)
  •     Template DNA
  •     Primers
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
1. DNA ligase cutting DNA, creating sticky ends of restriction fragments
2. DNA polymerase polymerase chain reaction to amplify sections of DNA
3. Electrophoresis separation of DNA fragments
4. Reverse transcriptase production of cDNA from mRNA
  • 1
  • 2
  • 3
  • 4
The source of the reverse transcriptase used in recombinant DNA technology, is a:     
 
  •     retrovirus
  •     plant cell
  •     cultured fungal cell
  •     cultured phage-infected mammalian cell
Consider the two statements:
I.    Agar Gel electrophoresis is useful in analysis of DNA. 
II.    DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole.
 
  •     Both I and II are correct and II explains I
  •     Both I and II are correct but II does not explain I
  •     I is correct but II is incorrect
  •     Both I and II are incorrect
Consider the given two statements:
I.    It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II.    When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
 
  •     Both I and II are correct and II explains I
  •     Both I and II are correct but II does not explain I
  •     I is correct but II is incorrect
  •     Both I and II are incorrect
Bacterial cells synthesize restriction endonucleases because restriction endonucleases help the bacterial cells:
 
  •     grow readily in medium containing antibodies.
  •     protect themselves from viral infection.
  •     open up the DNA in their chromosomes so that transcription is easier.
  •     take up foreign DNA from their environment and combine it into their genome.
Consider the following two statements:
I.    Plasmid can be used as a vector in recombinant DNA technology experiments.
II.    Plasmid is an extrachromosomal, circular, double stranded DNA found in some bacteria.
 
  •     Both I and II are correct and II explains I
  •     Both I and II are correct but II does not explain I
  •     I is correct but II is incorrect
  •     Both I and II are incorrect
What is true regarding Downstream Processing?
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
 
  • Only I
  • Only II
  • Both I and II
  • Neither I nor II
Identify the correct statement:
 
  •  Agrobacterium tumefaciens is able to transform large number of monocot plants.
  • Micro-injection is the only way to transform animal cells.
  • Competence in bacterial cells can be induced by trivalent anions like phosphates.
  • Biolistics or gene gun is a suitable method to transform plant cells.
What controls the copy number of linked DNA in a transformed bacterial cell?
 
  • Ori
  • Cloning sites
  • Selectable marker
  • Reporter gene
The fragments created by cutting DNA with restriction endonucleases can be separated by gel electrophoresis. What is incorrect regarding this procedure?
 
  • The fragments move towards anode.
  • Agar gel is used for sieving effect.
  • DNA fragments can be visualized moving in the medium if stained with ethidium bromide followed by exposure to UV radiation.
  • The larger fragments move farther in the medium.
The discipline of biotechnology was founded on the breakthrough work of:
 
  • Bolivar and Rodriquez
  • Cohen and Boyer
  • Nathans and Daniels
  • Redi and Spallanzani
In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
 
  • EcoR I
  • Hind II
  • Bam HI
  • Sma I
In a continuous culture system:
I. The used medium is drained out from one side while fresh medium is added from the other.
II. The cells are maintained in their physiologically most active log/exponential phase.
III. A larger biomass is produced leading to higher yields of desired proteins.
 
  • Only I and II are correct
  • Only I and III are correct
  • Only II and III are correct
  • I, II and III are correct
In Polymerase chain reaction:
  • the correct chronological sequence of steps is: Annealing of primer → Denaturation → Extension
  • Taq polymerase is an RNA polymerase derived from a fungus
  • millions to billions of copies of a specific DNA sample are made
  • viral antigens cannot be detected in a given sample
Which of the following statements will be correct regarding restriction endonucleases used in constructing recombinant DNA molecules?
  • They are RNA molecules acting as enzymes
  • The enzymes used in such experiments cut dsDNA randomly at any site
  • They have been isolated from bacterial cells
  • All restriction enzymes are thermostable and hence can be used in PCR procedures
DNA fragments created by the action of restriction enzymes can be separated by agar gel electrophoresis where:
I. larger the fragment size, the farther it moves in the gel
II. the DNA fragments are forced to move towards anode under an electric field
  • Only I is correct
  • Only II is correct
  • Both I and II are correct
  • Both I and II are incorrect
A ‘continuous culture system’ method usually produces a larger biomass mainly because:
  • the environmental conditions for the growth of the microbes are very easily maintained in such a system
  • such a system keeps most of the cells in their active/log exponential phase
  • it is very cheap with respect to other systems and hence cost of production is low
  • such a system uses larger sized bioreactors than other systems
Animal cells cannot be transformed with the help of:
  • Microinjection
  • Ti Plasmid
  • Retroviral vectors
  • Liposomes
The work of which of the following scientists was the basis upon which the discipline of biotechnology was founded?
  • Cohen and Boyer
  • Watson and Crick
  • Hershey and Chase
  • Avery, Macleod and McCarty
The DNA sequence shown below is:
5’ – GAATTC – 3’
3’ – CTTAAG – 5’              
 
  • the consensus sequence for prokaryotic promoter
  • the most common VNTR in human genome
  • the recognition sequence of a common restriction endonuclease EcoR I
  • an intron within eukaryotic split gene
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Practice Zoology Quiz Questions and Answers