tRNA looks like

  • clover-leaf

  • L shaped

  • inverted L shaped

  • a and c

The genetic make up of an organism is determined by

  • sequences of bases in RNA

  • sequence of bases in DNA

  • sequences of amino acids in proteins

  • all of these

HGP(Human Genome Project) gave a new area in biology called as

  • BioSciences

  • Bioinformatics

  • Biochemistry

  • Biological sciences

Which of the following was not the goal of Human Genome Project

  • Identification of human genes

  • human genome sequence determination

  • store information in databases

  • Improve manpower for data analysis

The Human Genome Project started in ______and completed, in__________ year respectively.

  • 1990, 2003

  • 1993, 2003

  • 1993, 2006

  • 1990, 2006

How many of the following were the features of the Human Genome

1. 99.9% bases are present are exactly same in all humans

2. all sequences were found to be coding sequences

3. Repeated sequences make up very large portion of the human genome

4. Repeated sequences are useful for chromosome structure, dynamics and evolution

5. The maximum no of genes were found to be present at the chromosome which was last sequenced

6. The fewest no of genes were found to be present at the chromosome which is present in male only.

  • 1

  • 2

  • 3

  • 5

The locations in human genome which differ by a single base difference is known as

  • Single nucleotide mutation

  • Single nucleotide polymorphism

  • RFLP

  • Single nucleotide polymorphogenesis

The largest known human gene is

  • Dystrophin protein gene on X chromosome

  • Sry gene on Y chromosome

  • Dystropin protein gene on Y chromosome

  • Sry gene on X chromosome

If an E.coli with heavy DNA allow to grow for 80 minutes in a culture medium of 14 NH 4

Cl, then what would be the proportion of heavy,light and hybrid densities DNA molecule

respectively?

  • 1/16 : 1/16 : 14/16

  • 0/16 : 14/16 : 2/16

  • 1/16 : 14/16 : 1/16

  • 1/8 : 2/8 : 6/8

Alec Jeffrey’s name is associated with

  • . DNA sequencing

  • . DNA fingerprinting

  • . RNA sequencing

  • . Gene cloning

Which of the following RNA polymerase catalyses the formation of transfer RNA in eukaryotes?

  • RNA polymerase I                        

  • RNA polymerase II

  • RNA polymerase I and II              

  • RNA polymerase III

Out of 64 codons how many of them code for amino acids?

  • 64                            

  • 20                            

  • 61                            

  • 32

Circular DNA is found in:

  • Viruses
  • Bacteria, chloroplast and mitochondria

  • Chloroplast and mitochondria alone

  • All of these

If in a DNA molecule cytosine is 18%, the percentage of adenine would be:

  • () 18%               
  • () 32%               
  • () 36%               
  • () 64%

Isotopes used in proving semiconservative replication of DNA were:

  • 14N 14C            

  • 14N 15N            

  • 14N 31P             

  • 14C 31P

Bacterial nucleoid has:

  • One single-stranded DNA            

  • One double-stranded DNA

  • Two single-stranded DNA             

  • Many double-stranded DNAs

Imagine an error occurring during DNA replication in a cell, so that where there is supposed to be a T in one of the genes there is instead a G. What effect will this probably have on the cell?

  • The amino acid sequence of one of its kinds of proteins will be completely changed
  • An amino acid will be missing from each of its kinds of proteins

  • One of its kinds of proteins might contain an incorrect amino acid

  • An amino acid will be missing from one of its kinds of proteins.

A sequence of pictures of polypeptide synthesis shows a ribosome holding two transfer RNAs. One tRNA has a polypeptide chain attached to it; the other tRNA has a single amino acid attached to it. What does the next picture show?

  • The polypeptide chain moves over and bonds to the single amino acid

  • The tRNA with the amino acid leaves the ribosome

  • The amino acid moves over and bonds to the polypeptide chain

  • The tRNA with the polypeptide chain leaves the ribosome

Column-I                                               Column-II
I. Genome of ϕ 174 bacteriophage           A. Ribozyme, RNAase
II. Purine                                               B. 5386 Nucleotides
III. Catalytic RNA                                   C. Adenine and Guanine
IV. Any chemical change in DNA              D. Mutation
The correct match is:

  • I - D, II - B, III - A, IV - C                 

  • I - B, II - A, III - D, IV - C

  • I - D, II - C, III - A, IV - B                 

  • I - B, II - C, III - A, IV - D

Column-I                                        Column-II
I. Operator site                               A. Binding site for RNA polymerase
II. Promoter site                              B. Binding site for repressor molecule
III. Structural gene                          C. Codes for enzyme protein
IV. Regulator gene                          D. Code for repressor molecules
The correct match is:

  • I - B, II - A, III - C, IV - D         

  • I - B, II - A, III - D, IV - C

  • I - D, II - C, III - A, IV - B         

  • I - B, II - C, III - A, IV - D

Column-I                           Column-II
I. Termination                    A. Aminoacyl tRNA synthetase
II. Translation                    B. Okazaki fragments
III. Transcription                C. GTP dependent release factor
IV. DNA replication            D. RNA polymerase
The correct match is:

  • I - B, II - A, III - C, IV - D           

  • I - C, II - A, III - D, IV - B

  • I - D, II - C, III - A, IV - B           

  • I - B, II - C, III - A, IV - D

In chromosome, DNA is associated with:

A. Negatively charged proteins       B. Positively charged proteins

C. Neutral proteins                        D. NHC protein

  • B, D                

    (2) A, B, C, D        

    (3) B, C, D            

    (4) A, B, C

  • 2
  • 3
  • 4

Heterochromatin:

A. Is more densely packed      B. Is stains dark

C. Is transcriptionally active    D. Is transcriptionally inactive

  • A, C                

  • A, B, C, D        

  • B, C, D            

  • A, B, D

In 1953, James Watson and F. Crick proposed the Double Helix model of DNA and got Nobel Prize. Their model of DNA was based on:
A. X-ray diffraction of DNA produced by M. Wilkins and R. Franklin
B. Griffith’s experiment.
C. Hershey - Chase experiment
D. Chargaff’s rule of base equivalence (A + G / T + C = 1)

  • A, D             
  • A, B, C, D           
  • B, C, D             
  • A, B, C

In one polynucleotide chain of a DNA molecule the ratio of A + T / G + C is 0.3. What is the A + G / T + C ratio of the entire DNA molecule:

  • 0.3                 

  • 0.6                 

  • 1.2                 

  • 1

DNA polymerase:
A. Is the main enzyme for RNA synthesis
B. Is DNA dependent DNA polymerizing enzyme
C. Is a highly efficient enzyme
D. Is catalyzes a reaction with a high degree of accuracy

  • C, D             

  • A, B, C, D               

  • B, C, D             

  • A, B, C

The difference(s) between mRNA and tRNA is / are that:
A. mRNA has more elaborated 3-dimensional structure due to extensive base-pairing.
B. tRNA has more elaborated 3-dimensional structure due to extensive pairing.
C. tRNA is usually smaller than mRNA.
D. mRNA bears anticodon but tRNA has codons.

  • A, C                   

  • A, B, C, D                 

  • B, C                   

  • A, B, C

In eukaryotes the gene expression is regulated at:
A. Transcriptional level
B. Processing level
C. Transport of mRNA from nucleus to the cytoplasm
D. Translational level

  • A, C               

  • A, B, C, D                 

  • B, C, D                     

  • A, B, C

In prokaryotes, gene regulation occurs at the level of:

  • Transcription                               

  • Translation

  • Post transcription                         

  • Post translation

Characteristic(s) of most DNA is / are:
A. A pairs with T by 2 hydrogen bonds.
B. Antipolarity of complementary chains
C. 20Aº diameter
D. 10 bps/turn 

  • A, C                 

  • A, B, C, D                     

  • B, C, D                 

  • A, B, C

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