Genetic information in a DNA molecule is coded in the:

  • Sequence of nucleotides

  • Base pairings 

  • Proportion of each base present

  • The turning pattern of the helix

In DNA replication, the Okazaki fragments on the lagging strand are joined together by:

  • DNA ligase

  • DNA polymerase

  • Primase

  • Helicase 

The enzyme that catalyzes the peptide bonding in prokaryotes is located in the:

  • Leader region of the mRNA

  • Central part of tRNA

  • Smaller subunit of the ribosome

  • Larger subunit of the ribosome

In general, bacterial genes are regulated at the time of:

  • Transcription

  • Post transcription

  • Translation

  • Post translation  

The insertion or deletion of a base pair into the genetic code will cause a frame shift mutation unless the number of base pairs inserted or deleted is:

  • One

  • Two

  • three

  • Ten

Consider the following statements:

I. The largest known human gene is dystrophin at 2.4 million bases

II. Repeated sequences make up very large portion of the human genome

III. There are about 2.4 million locations where single base DNA differences occur in humans

Which of the above statements are true?

  • I and II only

  • I and III only

  • II and III only

  • I, II and III

Consider the following statements:

I. RNA is the genetic material of the QB bacteriophage

II. DNA chemically is less reactive and more stable than RNA

III. Viruses having RNA genome and having shorter life span mutate & evolve slowly.

Which of the above statements are true?

  • I and II only

  • I and III only

  • II and III only

  • I, II and III

Consider the following statements:

I. Inheritance of a character is affected by promoter and regulatory sequences of a structural gene.
II. The split-gene arrangement represents an ancient feature of the genome.

III. The process of splicing represents the dominance of RNA world.
which of the above statements are true?

  • I and II only

  • I and III only

  • II and III only

  • I, II and III

Consider the following statements:

I. All the reference point while defining a transcription unit is made with coding strand

II. It is the presence of a promoter in a transcription unit that also defines the template and coding strands

III. Exons are said to be sequences that do not appear in mature or processed RNA

Which of the above statements are true?

  • I and II only

  • I and III only

  • II and III only

  • I, II and III

Consider the following statements:

I. Deoxyribonucleoside triphosphates act as substrates as well as provide energy for polymerization reaction during DNA replication.

II. The replication of DNA is both semiconsenvative and semi- discontinuous

III. In bacterial DNA replication there are multiple ori and replication fork moves bi-directionally
Which of the above statements are true?

  • I and II only

  • I and III only

  • II and III only

  • I, II and III

Match each item in Column I with one item in Column II and chose your answer from the codes given below:

Column I

(nRNA codons)

Column II

(Amino Acid)

I. GUG

II. UGG

III. UAC

IV. GAG

1. Tryptophan

2. Valine

3. Glutamic acid

4. Tyrosine

 

Codes

             I           II         III         IV

  •          1           2           3           4

  •          2           1           4           3

  •          2           1           3           4

  •          1           2           4           3

If there are 999 bases in an RNA that codes for a protein with 333 amino acids and the base at position 901 is deleted such that the length of teh RNA becomes 998 bases, how many codons will be altered?

  • 1

  • 11

  • 33

  • 333

DNA fragments are

  • positively charged

  • negatively charged

  • neutral

  • either positively or negatively charged depending on their size

During DNA replication, Okazaki fragments are used to elongate

  • The leading strand towards replication fork

  • The lagging strand towards replication fork

  • The leading strand away from replication fork

  • (2) The lagging strand away from replication fork

Spliceosomes are not found in cells of 

  • plants

  • fungi

  • animals 

  • bacteria

The final proof for DNA as the genetic material come from the experiments of 

  • Griffith

  • Hershey and chase 

  • Avery, Macleod and McCarty 

  • Hargobind Khorana 

The association of histone H1 with a nucleosome indicates 

  • transcription is occuring

  • DNA replication is occuring

  • the DNA is condensed into chromatin fibre

  • the DNA double helix is exposed

Which of the following RNAs should be most abundant in animals cell ?

  • rRNA

  • tRNA

  • mRNA

  • miRNA

Taylor conducted the experiments to prove semi-conservative mode of chromosome replication on

  •  Vinca rosea

  • Vicia faba

  • Drosophila melanogaster

  • E. coli

The equivalent of a structural gene is

  • muton

  • cistron

  • operon

  • recon

How many types of nitrogenous bases are present in nucleic acid

  • 4

  • 3

  • 5

  • 6

Select the correct option 

 

 Direction of RNA synthesis 

 Direction of reading of the template DNA strand 

(1)

5'-3'

3'-5'

(2)

3'-5'

5'-3'

(3)

5'-3'

5'-3'

(4)

3'-5'

3'-5'

 

 

 

  • 1
  • 2
  • 3
  • 4

Commonly used vectors for human genome sequencing are

  • T-DNA

  • BAC and YAC

  • Expression vectors

  • T/A cloning vectors

Which enzymes/s will be produced in a cell in which there is a non-sense mutation in the lac Y gene?

  • β-galactosidase

  • Lactose permease

  • Transacetylase

  • Lactose permease and transacetylase

Removal of RNA polymerase III from nucleoplasm will affect the synthesis of

  • tRNA

  • hnRNA

  • mRNA

  • rRNA

PCR and Restriction Fragment Length Polymorphism are the methods for

  • study of enzymes

  • genetic transformation

  • DNA sequencing

  • genetic fingerprinting

Which one of the following is not a part of a transcription unit in DNA?

  • The inducer

  • A terminator

  • A promoter

  • The structural gene

If one strand of DNA has the nitrogenous base sequence as ATCTG, what would be the complementary RNA strand sequence?

  • TTAGU

  • UAGAC

  • AACTG

  • ATCGU

Removal of introns and joining of exons in a defined order during transcription is called

  • looping

  • inducing

  • capping

  • splicing

What are those structures that appear as ‘beads-on-string’ in the chromosomes when viewed under electron microscope?

  • Nucleotides

  • Nucleosomes

  • Base pairs

  • Genes

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